Recovery of Vibrio parahaemolyticus strains inoculated on the shrimp Litopenaeus vannamei submitted to cooling and freezing temperatures

Authors

  • Dannielle Batista Rolim Sousa Universidade Federal do Ceará.
  • Antonio Adauto Fonteles-Filho Instituto de Ciências do Mar, Universidade Federal do Ceará.
  • Oscarina Viana de Sousa Instituto de Ciências do Mar, Universidade Federal do Ceará.
  • Regine Helena Silva dos Fernandes Vieira Instituto de Ciências do Mar, Universidade Federal do Ceará.
  • Camila Magalhães Silva Instituto de Ciências do Mar, Universidade Federal do Ceará.

DOI:

https://doi.org/10.32360/acmar.v44i2.163

Keywords:

Vibrio parahaemolyticus, strains, inoculation, shrimp, Litopenaeus vannamei, temperature effect

Abstract

The aim of this study was to evaluate the survival of Vibrio parahaemolitycus inoculated in shrimp Litopenaeus vannamei at different temperatures of refrigeration (refrigerator, freezer and isothermic box with ice) during ten days and on the 15th, 20th and 25th days. The experiment was repeated six times from October, 2005 to March, 2006. Shrimps were obtained on a fish market located at Praia do Mucuripe, Fortaleza, Ceará. In the laboratory, the shrimps were washed with distilled water and immersed in boiling water for five minutes in order to eliminate any other Vibrio species in the sample. The inoculation happened with the contact between the shrimps and the V. parahaemolyticus culture for five minutes. Then, the sample was divided in forty 25-g portions, one of which was used as initial time. The other 39 portions were separated in three batches maintained on freezer (-21°C), refrigerator (11°C) and isothermic box with ice (-1°C to 13°C). The number of V. parahaemolyticus was monitored for 10 days and on 15th, 20th and 25th days by the plate count method on TCBS and PCA media. All temperatures were efficient to control the viable cells of V. parahaemolyticus. The action of cold produced by ice, refrigerator and freezer inhibit the bacterial growth on shrimps. The freezer was the most efficient treatment in reducing the action of V. parahaemolyticus on the shrimp L. vannamei.

Author Biographies

Dannielle Batista Rolim Sousa, Universidade Federal do Ceará.

Estudante do Programa de Pós-Graduação em Engenharia de Pesca.

Antonio Adauto Fonteles-Filho, Instituto de Ciências do Mar, Universidade Federal do Ceará.

Professor e pesquisador.

Oscarina Viana de Sousa, Instituto de Ciências do Mar, Universidade Federal do Ceará.

Professor e pesquisador.

Regine Helena Silva dos Fernandes Vieira, Instituto de Ciências do Mar, Universidade Federal do Ceará.

Professor e pesquisador.

Camila Magalhães Silva, Instituto de Ciências do Mar, Universidade Federal do Ceará.

Estudante do Programa de Ciências Marinhas Tropicais

Published

2011-09-01

Issue

Section

Artigos originais